AFG3L2 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-AFG3L2 Antibody (A329102) at a dilution of 1:1000 and detected with chemiluminescence.
AFG3L2 expression in human colon carcinoma tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-AFG3L2 Antibody (A329102) at a dilution of 1:200.
AFG3L2 expression in 600ug extracts of Mouse liver analyzed by western blot following immunoprecipitation, both using Anti-AFG3L2 Antibody (A329102) at 3ug and a dilition of 1:500, respectively.
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