Anti-AFG3L2 Antibody (A91394)

Name: Anti-AFG3L2 Antibody
Brand: Antibodies.com
SKU: A91394
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to AFG3L2 for WB, IHC and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-AFG3L2 Antibody

Description

Rabbit polyclonal antibody to AFG3L2.

Applications

WB, IHC, IP

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 538-797 of human AFG3L2 (NP_006787.2).

Sequence

DSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

80 - 90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

AFG3-like protein 2, Mitochondrial inner membrane m-AAA protease component AFG3L2, Paraplegin-like protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-AFG3L2 Antibody (A91394)

Western blot analysis of various lysates, using Anti-AFG3L2 Antibody (A91394) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-AFG3L2 Antibody (A91394)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-AFG3L2 Antibody (A91394) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Western Blot - Anti-AFG3L2 Antibody (A91394)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-AFG3L2 Antibody (A91394). This Western blot was performed on the immunoprecipitate using Anti-AFG3L2 Antibody (A91394) at a dilution of 1:1000.

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