Anti-AIF1 Antibody (A320074)

Anti-AIF1 Antibody

Goat polyclonal antibody to AIF1.

This antibody has been used successfully for the identification of AIF1 expressing cells in an inflammatory model in formalin fixed mouse brain following heat mediated antigen retrieval with sodium citrate buffer by immunohistochemistry.

WB, IHC-P, ELISA

ELISA: 1:32,000, IHC-P: 2 µg/ml, WB: 1 - 3.0 µg/ml

Human, Mouse, Pig, Rat

Synthetic peptide sequence C-TGPPAKKAISELP from the C-terminus of AIF1 (NP_116573.1; NP_001614.3).

C-TGPPAKKAISELP

Goat

Polyclonal

IgG

Approximately 16 kDa in rat brain cell lysates.

Liquid

Supplied in TRIS Buffered Saline, 0.5% BSA and 0.02% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Human allograft inflammatory factor 1 (AIF1) is otherwise known as Ionized calcium-binding adapter molecule 1 (IBA1). AIF1 is a 147 amino acid ~17kDa actin-binding protein involved in macrophage activation, vascular smooth muscle cell proliferation and activation and T-lymphocyte proliferation (UniProt P55008). Goat anti AIF1 antibody recognizes epitopes at the C-terminal region of AIF1 and is expected to recognize AIF1 isoform 1 and isoform 3 formed by alternative splicing.AIF1 was first identified in rat cardiac allografts with chronic rejection (Utans et al. 1995), is expressed by activated monocytes and macrophages (Orsmark et al. 2007) and plays a significant role in vascular inflammation (Chen et al. 2004).

AIF-1, Allograft inflammatory factor 1, G1, Ionized calcium-binding adapter molecule 1, Protein G1