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Anti-Akt (Ab-129) Antibody (A34732)

Immunohistochemistry - Akt (Ab-129) Antibody from Signalway Antibody (33145) - Antibodies.com Immunofluorescence - Akt (Ab-129) Antibody from Signalway Antibody (33145) - Antibodies.com Western blot - Akt (Ab-129) Antibody from Signalway Antibody (33145) - Antibodies.com Western blot - Akt (Ab-129) Antibody from Signalway Antibody (33145) - Antibodies.com
This product is discontinued

Anti-Akt (Ab-129) Antibody (A34732) has been discontinued and is no longer available.

View all Akt Antibodies.

  • Specifications

Name
Anti-Akt (Ab-129) Antibody
Description
Rabbit polyclonal antibody to Akt (Ab-129)
Specificity
The antibody detects endogenous levels of total Akt protein.
Applications
WB, IHC, IF
Reactivity
Human
Immunogen
Synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of serine 129 (D-N-S(p)-G-A).
Host
Rabbit
Clonality
Polyclonal
Conjugate

Unconjugated

Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Immunohistochemistry - Akt (Ab-129) Antibody from Signalway Antibody (33145) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Akt (Ab-129) Antibody (A34732)

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Akt (Ab-129) antibody #33145.
Immunofluorescence - Akt (Ab-129) Antibody from Signalway Antibody (33145) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Akt (Ab-129) Antibody (A34732)

Immunofluorescence analysis of HeLa cells, using Akt (Ab-129) antibody #33145.
Western blot - Akt (Ab-129) Antibody from Signalway Antibody (33145) - Antibodies.comEnlarge Image

Anti-Akt (Ab-129) Antibody (A34732)

Western blot analysis of extracts from HuvEc cells, treated with Serum (30%, 30mins), using Akt (Ab-129) antibody #33145.
Western blot - Akt (Ab-129) Antibody from Signalway Antibody (33145) - Antibodies.comEnlarge Image

Anti-Akt (Ab-129) Antibody (A34732)

Western blot analysis of extracts from HuvEc cells (Lane 2) and JK cells (Lane 3), using Akt (Ab-129) antibody #33145. The lane on the left is treated with synthesized peptide.

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