Rabbit polyclonal antibody to AKT1 / 3 (Phospho-Tyr437 / 434)
Specificity
The antibody detects endogenous levels of AKT1 / 3 only when phosphorylated at tyrosine 437.
Applications
WB, IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 437 (T-R-Y(p)-F-D) derived from Human AKT1 / 3.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C
Synonyms
PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha
Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using AKT1/3 (Phospho-Tyr437/434) antibody #12116. The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using AKT1/3 (Phospho-Tyr437/434) antibody #12116. The picture on the right is treated with the synthesized peptide.
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