Rabbit polyclonal antibody to ALK (Phospho-Tyr1604)
Specificity
The antibody detects endogenous levels of ALK only when phosphorylated at tyrosine 1604.
Applications
WB, IHC, IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 1604 (G-H-Y(p)-E-D) derived from Human ALK.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HepG2 cells, using ALK (Phospho-Tyr1604) antibody #12127. The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from cos-7 cells (Lane 2), using ALK (Phospho-Tyr1604) Antibody #12127. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ALK (Phospho-Tyr1604) antibody #12127. The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using ALK (Phospho-Tyr1604) antibody #12127. The picture on the right is treated with the synthesized peptide.
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