Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Alpha(B)-crystallin, Alpha-crystallin B chain, CRYA2, CRYAB, Heat shock protein beta-5, Heat shock protein family B member 5, HSPB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component
Western Blot - Anti-Alpha B Crystallin Antibody (A88702)
Western blot analysis of extracts of various cell lines, using Anti-Alpha B Crystallin Antibody (A88702) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
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