Rabbit polyclonal antibody to CRYAB (phospho Ser45).
Specificity
This antibody detects endogenous levels of CRYAB only when phosphorylated at Ser45.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human CRYAB around the phosphorylation site of Ser45 (amino acids 21-70).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
20kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Alpha(B)-crystallin, Alpha-crystallin B chain, CRYA2, CRYAB, Heat shock protein beta-5, Heat shock protein family B member 5, HSPB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component
Immunohistochemical analysis of paraffin-embedded human heart using Anti-CRYAB (phospho Ser45) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-CRYAB (phospho Ser45) Antibody (A94452)
Western blot analysis of lysates from COS7 cells treated with anisomycin 25ug/ml 30' using Anti-CRYAB (phospho Ser45) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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