Western Blot - Anti-Alpha-synuclein (phospho Tyr136) Antibody (A16546)
Western blot analysis of extracts of Mouse brain, using Anti-Alpha-synuclein (phospho Tyr136) Antibody (A16546) at 1:1,000 dilution. Mouse brain were treated by λ-PP mixed solution (1ul) at 30°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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