Anti-alpha Tubulin Antibody (A304857)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of 50.1 kDa alpha Tubulin Antibody protein using Anti-alpha Tubulin Antibody (A304857) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: human HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat-Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: TMB. Predicted/Observed Size: 50.1 kDa.

Western blot analysis of human lung carcinoma epithelial cell line (A549) lysate showing detection of 50.1 kDa alpha Tubulin Antibody protein using Anti-alpha Tubulin Antibody (A304857) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: human A549 cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 50.1 kDa.

Immunocytochemistry/Immunofluorescence analysis of mouse NIH 3T3 (mouse fibroblast cell line), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-alpha Tubulin Antibody (A304857), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm, Cytoskeleton. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) alpha Tubulin Antibody (D) Composite.