Anti-ARFGAP1 Antibody (A89816)

This product is discontinued

Anti-ARFGAP1 Antibody (A89816) has been discontinued and is no longer available.

View all ARFGAP1 Antibodies.

Specifications

Name

Anti-ARFGAP1 Antibody

Description

Rabbit polyclonal antibody to ARFGAP1.

Applications

WB, IHC, IF

Dilutions

WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ARFGAP1 (NP_001268411.1).

Sequence

MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESSPAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

42kDa

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADP-ribosylation factor 1 GTPase-activating protein, ADP-ribosylation factor GTPase-activating protein 1, ARF GAP 1, ARF1 GAP, ARF1-directed GTPase-activating protein, ARF1GAP

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

Western Blot - Anti-ARFGAP1 Antibody (A89816)

Western blot analysis of extracts of various cell lines, using Anti-ARFGAP1 Antibody (A12595) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.

Immunohistochemistry - Anti-ARFGAP1 Antibody (A89816)

Immunohistochemistry of paraffin-embedded rat brain using Anti-ARFGAP1 Antibody (A12595) at dilution of 1:100 (40x lens).

Immunohistochemistry - Anti-ARFGAP1 Antibody (A89816)

Immunohistochemistry of paraffin-embedded human gastric cancer using Anti-ARFGAP1 Antibody (A12595) at dilution of 1:100 (40x lens).

Immunofluorescence - Anti-ARFGAP1 Antibody (A89816)

Immunofluorescence analysis of A549 cells using Anti-ARFGAP1 Antibody (A12595). Blue: DAPI for nuclear staining.

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See all ARFGAP1 Antibodies

Proteins predicted to interact with ARFGAP1

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.

ARF1 Antibodies

98.9% Relevancy Score

GCS1 Antibodies

96% Relevancy Score

ZNF289 Antibodies

89.9% Relevancy Score

ARF6 Antibodies

89% Relevancy Score

ARFGAP3 Antibodies

88.9% Relevancy Score

GBF1 Antibodies

80.9% Relevancy Score

ARF5 Antibodies

79.5% Relevancy Score

AP2B1 Antibodies

76.8% Relevancy Score

EDC4 Antibodies

76.7% Relevancy Score