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Anti-AurB (phospho Thr232) Antibody (A93646)

Immunofluorescence - Anti-AurB (phospho Thr232) Antibody (A1021) - Antibodies.com Immunohistochemistry - Anti-AurB (phospho Thr232) Antibody (A1021) - Antibodies.com Western Blot - Anti-AurB (phospho Thr232) Antibody (A1021) - Antibodies.com
This product is discontinued

Anti-AurB (phospho Thr232) Antibody (A93646) has been discontinued and is no longer available.

View all Aurora B Antibodies.

  • Specifications

Name
Anti-AurB (phospho Thr232) Antibody
Description
Rabbit polyclonal antibody to AurB (phospho Thr232).
Specificity
This antibody detects endogenous levels of AurB only when phosphorylated at Thr232.
Applications
WB, IHC, IF, ELISA
Dilutions
WB: 1:500~1:1000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:20000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human AurB around the phosphorylation site of Thr232 (amino acids 198-247).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
39kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AIK2, AIM-1, AIM1, AIRK2, ARK-2, ARK2, AURKB, Aurora 1, Aurora kinase B, Aurora- and IPL1-like midbody-associated protein 1, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, Serine/threonine-protein kinase 12, STK-1, STK1, STK12, STK5
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Immunofluorescence - Anti-AurB (phospho Thr232) Antibody (A1021) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-AurB (phospho Thr232) Antibody (A93646)

Immunofluorescence analysis of HepG2 cells using Anti-AurB (phospho Thr232) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunohistochemistry - Anti-AurB (phospho Thr232) Antibody (A1021) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-AurB (phospho Thr232) Antibody (A93646)

Immunohistochemical analysis of paraffin-embedded human liver carcinoma using Anti-AurB (phospho Thr232) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-AurB (phospho Thr232) Antibody (A1021) - Antibodies.comEnlarge Image

Western Blot - Anti-AurB (phospho Thr232) Antibody (A93646)

Western blot analysis of lysates from COS7 cells treated with Nocodazole 1µg/ml 16h using Anti-AurB (phospho Thr232) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Proteins predicted to interact with Aurora B

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score
99.7% Relevancy Score
99.7% Relevancy Score
99.7% Relevancy Score
99.6% Relevancy Score