Rabbit polyclonal antibody to Aurora A (phospho-Thr288)
Specificity
The antibody detects endogenous level of Aurora A only when phosphorylated at threonine 288.
Applications
WB, IHC
Dilutions
Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of threonine 288 (R-T-T(p)-L-M) derived from Human Aurora A.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
48kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemistry - Anti-Aurora A (phospho-Thr288) Antibody (A50385)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Aurora A (Phospho-Thr288) Antibody #11519 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HT29 cells, treated with Hydroxyurea or calf intestinal phosphatase (CIP), using Aurora A (phospho-Thr288) Antibody #11519.
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