Immunohistochemistry analysis of human breast, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Axin 1 Antibody (A305162) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Western blot analysis of rat kidney showing detection of ~95 kDa Axin-1 protein using Anti-Axin 1 Antibody (A305162) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat Kidney. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~95 kDa.
Immunohistochemistry analysis of human breast, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Axin 1 Antibody (A305162) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
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