Rabbit polyclonal antibody to B-RAF (Phospho-Thr599)
Specificity
The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 598.
Applications
IHC
Dilutions
Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of threonine 599 (L-A-T(p)-V-K) derived from Human B-RAF.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using B-Raf (Phospho-Thr598) antibody #11682 (left)or the same antibody preincubated with blocking peptide (right).
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