B7-H3 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-B7-H3 Antibody (A329142) at a dilution of 1:2000 and detected with chemiluminescence. Negative control: Jurkat cells
B7-H3 expression in human placenta tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-B7-H3 Antibody (A329142) at a dilution of 1:200.
B7-H3 expression in human liver cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-B7-H3 Antibody (A329142) at a dilution of 1:200.
B7-H3 expression in human thyroid cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-B7-H3 Antibody (A329142) at a dilution of 1:200.
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