Anti-Bax Antibody [ARC5006-10] (A306010)

Western blot analysis of various lysates, using Anti-Bax Antibody [ARC5006-10] (A306010) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts from wild type(WT) and Bax knockout (KO) 293T cells, using Anti-Bax Antibody [ARC5006-10] (A306010) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded rat lymph node using Anti-Bax Antibody [ARC5006-10] (A306010) at a dilution of 1:20，1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Bax Antibody [ARC5006-10] (A306010) at a dilution of 1:20，1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.