Western Blot - Anti-beta Catenin (phospho Ser552) Antibody [ARC54001] (A308575)
Western blot analysis of extracts of various cell lines, using Anti-beta Catenin (phospho Ser552) Antibody [ARC54001] (A308575) at 1:1,000 dilution. 293T cells were treated by λ-PP mixed solution (1ul) at 30°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western Blot - Anti-beta Catenin (phospho Ser552) Antibody [ARC54001] (A308575)
Western blot analysis of extracts of various cell lines, using Anti-beta Catenin (phospho Ser552) Antibody [ARC54001] (A308575) at 1:1,000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
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