Rabbit polyclonal antibody to beta-catenin (Phospho-Ser715)
Specificity
The antibody detects endogenous level of beta-catenin only when phosphorylated at serine 715.
Applications
WB, IHC
Dilutions
Western blotting:1:500~1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 715 (D-P-S(p)-Y-R) derived from Human beta-catenin.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical analysis of paraffin-embedded human primary glioblastoma multiform (GBM) specimens using beta-catenin (Phospho-Ser715) Antibody #11594.
Western blot analysis of extract from U87 cells transfected with either PKCd siRNA targeting or control siRNA were treated withWnt3a (100 ng ml 1) for 8 h. WB was performed with nuclear lysates of the cells with the beta-catenin (Phospho-Ser715) Antibody #11594.
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