Bmi1 expression in K562 nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Bmi1 Antibody (A85203) at 0.03µg/ml and detected by chemiluminescence.
Bmi1 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Bmi1 Antibody (A85203) at 5µg/ml and revealed with alkaline phosphatase (AP).
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