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Anti-BRG1 Antibody (A375)

Identification of Brg1 in whole cell extracts of human and mouse cells by western blotting using anti-Brg1 antibody. Lane 1; Protein size makers (kDa) Lane 2; HeLa (human) cell extract Lane 3; NIH 3T3 (mouse) cell extract *Star indicates the position of Brg1 protein bands (Predicted molecular mass of Bgr1 is 185 kDa). Anti-Brg1 antibody was used at 1/1,000 dilution Immunoprecipitation of Brg1 protein from nuclear extracts of HeLa cells by using anti-Brg1 antibody. Brg1 was precipitated with anti-Brg1 antibody and proteinG-conjugated agarose beads and probed with anti-Brg1 antibody by western blotting.. Lane1; Input nuclear extracts Lane 2; Control IP with non-immune IgG Lane 3; Immunoprecipitation with anti-Brg1 antibody Immunofluorescence staining of Brg1 protein in HeLa cells. Hela cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in PBS for 10 min. Anti-Brg1 antibody was used at 1/1,000 dilution. As second antibody, goat anti-rabbit IgG conjugated with Alex488 was used at 1/1,000 dilution. Nuclei were stained with DAPI. Brg1 protein is localized in nuclei. Identification of Brg1 in whole cell extracts of human and mouse cells by western blotting using anti-Brg1 antibody. Immunoprecipitation of Brg1 protein from nuclear extracts of HeLa cells by using anti-Brg1 antibody. Immunofluorescence staining of Brg1 protein in HeLa cells.
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$560
Product Datasheet

Rabbit polyclonal antibody to BRG1 for WB, IP and IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-BRG1 Antibody
Description
Rabbit polyclonal antibody to BRG1.
Applications
WB, IP, IF
Dilutions
WB: 1:1,000, IF: 1:1,000
Reactivity
Human, Mouse
Immunogen
Synthetic peptide corresponding to the C-terminal of human BRG1 protein, conjugated with KLH.
Sequence
C-KLGRKEKAQDRLKGGRRRPSRGSRAKPVVSDDDSEEEQEEDRSGSGSEED
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ATP-dependent helicase SMARCA4, BAF190A, BRG1-associated factor 190A, Mitotic growth and transcription activator, Protein brahma homolog 1, Protein BRG-1, SMARCA4, SNF2B, SNF2L4, Transcription activator BRG1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (6)

Identification of Brg1 in whole cell extracts of human and mouse cells by western blotting using anti-Brg1 antibody. Lane 1; Protein size makers (kDa) Lane 2; HeLa (human) cell extract Lane 3; NIH 3T3 (mouse) cell extract *Star indicates the position of Brg1 protein bands (Predicted molecular mass of Bgr1 is 185 kDa). Anti-Brg1 antibody was used at 1/1,000 dilutionEnlarge Image

Anti-BRG1 Antibody (A375)

Anti-BRG1 Antibody
Immunoprecipitation of Brg1 protein from nuclear extracts of HeLa cells by using anti-Brg1 antibody. Brg1 was precipitated with anti-Brg1 antibody and proteinG-conjugated agarose beads and probed with anti-Brg1 antibody by western blotting.. Lane1; Input nuclear extracts Lane 2; Control IP with non-immune IgG Lane 3; Immunoprecipitation with anti-Brg1 antibodyEnlarge Image

Immunoprecipitation - Anti-BRG1 Antibody (A375)

Anti-BRG1 Antibody
Immunofluorescence staining of Brg1 protein in HeLa cells. Hela cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in PBS for 10 min. Anti-Brg1 antibody was used at 1/1,000 dilution. As second antibody, goat anti-rabbit IgG conjugated with Alex488 was used at 1/1,000 dilution. Nuclei were stained with DAPI. Brg1 protein is localized in nuclei.Enlarge Image

Immunofluorescence - Anti-BRG1 Antibody (A375)

Anti-BRG1 Antibody
Identification of Brg1 in whole cell extracts of human and mouse cells by western blotting using anti-Brg1 antibody. Enlarge Image

Anti-BRG1 Antibody (A375)

Anti-BRG1 Antibody
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Proteins predicted to interact with BRG1

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score