Western Blot - Anti-BTK (phospho Tyr223) Antibody [ARC0307] (A305366)
Western blot analysis of extracts of Raji cells, using Anti-BTK (phospho Tyr223) Antibody [ARC0307] (A305366) at 1:500 dilution. Raji cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
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