Dilutions
Flow Cytometry: 0.5-1 µg/million cells, IF: 1-2 µg/mL, IHC-P: 1-2 µg/mL for 30 minutes at room temperature. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM Tris buffer containing 1 mM EDTA, pH 9.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.