Anti-C5aR Antibody (A334482)

Detection of Complement C5a receptor 1 (C5aR) by immunohistochemistry in mouse spleen. Mouse spleen sections were dewaxed, microwaved in citric acid, and incubated with anti-C5aR antibody (A334482) at 1:100. Sections were treated with biotinylated anti-rabbit IgG and avidin-biotin solution, developed with 3-amino-9-ethylcarbazole (AEC), and counterstained with hematoxylin. C5aR receptors were observed at the plasma membrane of nearly all cells in the spleen.

Localization of Complement C5a receptor 1 (C5aR) in mouse lung by immunohistochemistry. Lung sections were dewaxed, microwaved in citric acid, and stained with anti-C5aR antibody (A334482) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. C5aR receptors were uniformly detected at the plasma membrane of lung cells.

Western blot analysis of Complement C5a receptor 1 (C5aR) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing mouse C5aR were immunoblotted with the phosphorylation-independent anti-C5aR antibody (A334482) at a 1:1000 dilution.