Calbindin expression in Human Kidney lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Calbindin Antibody (A83719) at 0.3µg/ml and detected by chemiluminescence.
Calbindin expression in Rat Brain (A) and Kidney (B) lysate, analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-Calbindin Antibody (A83719) at 0.3µg/ml (A) or 0.1µg/ml (B) and detected by chemiluminescence.
Calbindin expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-Calbindin Antibody (A83719) at 5µg/ml and revealed with horseradish peroxidase (HRP).
Negative control for Calbindin expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
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