Anti-Caldesmon Antibody (A250386)

Anti-Caldesmon Antibody

Rabbit polyclonal antibody to Caldesmon.

This antibody recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This antibody This antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

WB, Flow Cytometry, IF, IHC

WB: 1-2 µg/ml, Flow Cytometry: 0.5-1 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human, Rat

Recombinant fragment of human CALD1 protein.

Rabbit

Polyclonal

IgG

Protein A/G chromatography.

200 µg/ml

120-150 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Caldesmon Antibody - BSA and Azide free (A253566).

CAD, CALD1, CDM