Anti-Calretinin Antibody [3G9] (A85367)

Immunofluorescent analysis of rat cerebellum section stained with Anti-Calretinin Antibody, at a dilution of 1:500, in green, and Anti-Calbindin Antibody (A85359 | 1:2,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The Anti-Calretinin Antibody labels interneurons in the granule cell layer, while the Anti-Calbindin Antibody stains Purkinje neurons and their dendritic processes in the molecular layer of the cerebellum.

Left: Adult mouse brain hippocampal section (45 µM; fixed by transcardial perfusion with 4% paraformaldehyde) was co-stained with Anti-Calretinin Antibody, at a dilution of 1:1000, in red, and Anti-Fox3/NeuN Antibody (A85403 | 1:1,000, in green. Anti-Calretinin Antibody stains a small number of interneuron in the stratum radiatum of CA1 region, and Fox/NeuN is expressed in most neurons in the brain. As a result, cells are positive for calretinin appear to be yellow. Right: Adult rat cortical section (45 µM; fixed by transcardial perfusion with 4% paraformaldehyde) was co-stained with Anti-Calretinin Antibody (red) and Anti-Calbindin Antibody (A85359 | green). In cortex, calretinin is expressed in a small population of interneurons concentrated in Layer 4 area, while calbindin is expressed in cells concentrated in Layer 2/3. Because each antibody specifically labels a different population of cells exclusively, the cells are either stained with green or red in the cortex. Insets are high-magnification images of the boxed area in each picture. Blue is DAPI staining that labels DNA.

Immunohistochemistry analysis of a NBF fixed paraffin embedded human brain cortex section with Anti-Calretinin Antibody [3G9] (A85367) at a dilution of 1:2,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). Anti-Calretinin Antibody [3G9] (A85367) strongly stains the nucleus and cytoplasm of pyramidal neurons. The Anti-Calretinin Antibody [3G9] (A85367) performs well in testing with both 4% PFA and standard NBF fixed tissues but does not stain long term NBF fixed tissue effectively.

Western blot analysis of recombinant protein solutions using Anti-Calretinin Antibody [3G9] (A85367), at a dilution of 1:1,000, in green. The lanes contain samples of: [Lane 1] Protein standards, in red, [Lane 2] calbindin, [Lane 3] calretinin, [Lane 4] parvalbumin, and [Lane 5] secretogogin. The Anti-Calretinin Antibody [3G9] (A85367) reacts only with calretinin, and does not bind to other related calcium-binding proteins.