Anti-Calretinin Antibody (A104312)

Immunofluorescent analysis of rat hippocampus section stained with Anti-Calretinin Antibody (A104312), at a dilution of 1:1,000, in green, and co-stained with Anti-Parvalbumin Antibody [3C9] (A85317), at a dilution of 1:1,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The Anti-Calretinin Antibody (A104312) and Anti-Parvalbumin Antibody [3C9] (A85317) label different classes of interneurons.

Western blot analysis of different tissue lysates using Anti-Calretinin Antibody (A104312), at a dilution of 1:1,000, in green. The lanes contain: [Lane 1] protein standard (red), [Lane 2] rat brain, [Lane 3] rat spinal cord, [Lane 4] mouse brain, [Lane 5] mouse spinal cord, and [Lane 6] cow spinal cord. A band at 29kDa corresponds to Calretinin protein. The same blot was simultaneously probed with Anti-alpha Internexin Antibody [2E3] (A85448), at a dilution of 1:10,000, in red, that reveals the alpha Internexin protein with apparent molecular weight of 66kDa.

Immunohistochemistry analysis of a formalin fixed paraffin embedded rat brain section with Anti-Calretinin Antibody (A104312) at a dilution of 1:2,000 detected in DAB (brown) following the ABC method. Counterstained with Hematoxylin (blue). The subfornical organ region is imaged, showing strong labeling of dorsolateral peripheral neurons (9).