The antibody detects endogenous levels of total CARM1 protein.
Applications
WB, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around aa.226~230 (V-K-S-N-N) derived from CARM1
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min), using CARM1 (Ab-228) antibody (#21331, Line 1 and 2) and CARM1 (Phospho-Ser228) antibody (#11331, Line 3 and 4).