Anti-CARM1 Antibody (A13966)

Name: Anti-CARM1 Antibody
Brand: Antibodies.com
SKU: A13966
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit polyclonal antibody to CARM1 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-CARM1 Antibody

Description

Rabbit polyclonal antibody to CARM1.

Applications

WB, IHC

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 486-603 of human PRMT4/CARM1 (NP_954592.1).

Sequence

GSHYTSPSENMWNTGSTYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGSGGGSTSAHYAVNSQFTMGGPAISMASPMSIPTNT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

63 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Coactivator-associated arginine methyltransferase 1, Histone-arginine methyltransferase CARM1, PRMT4, Protein arginine N-methyltransferase 4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-CARM1 Antibody (A13966)

Western blot analysis of extracts from normal (control) and PRMT4/CARM1 knockout (KO) 293T cells, using Anti-CARM1 Antibody (A13966) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-CARM1 Antibody (A13966)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-CARM1 Antibody (A13966) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-CARM1 Antibody (A13966)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-CARM1 Antibody (A13966) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-CARM1 Antibody (A13966)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-CARM1 Antibody (A13966) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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