Anti-CBL (phospho Tyr774) Antibody (A93786)

Name: Anti-CBL (phospho Tyr774) Antibody
Brand: Antibodies.com
SKU: A93786
Price: 115.0 GBP
Availability: InStock

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This product is discontinued

Anti-CBL (phospho Tyr774) Antibody (A93786) has been discontinued and is no longer available.

View all CBL Antibodies.

Specifications

Name

Anti-CBL (phospho Tyr774) Antibody

Description

Rabbit polyclonal antibody to CBL (phospho Tyr774).

Specificity

This antibody detects endogenous levels of CBL only when phosphorylated at Tyr774.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500~1:1000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:1000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human CBL around the phosphorylation site of Tyr774 (amino acids 740-789).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

99kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Casitas B-lineage lymphoma proto-oncogene, CBL2, E3 ubiquitin-protein ligase CBL, Proto-oncogene c-Cbl, RING finger protein 55, RING-type E3 ubiquitin transferase CBL, RNF55, Signal transduction protein CBL

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Immunofluorescence - Anti-CBL (phospho Tyr774) Antibody (A93786)

Immunofluorescence analysis of A549 cells using Anti-CBL (phospho Tyr774) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Immunohistochemistry - Anti-CBL (phospho Tyr774) Antibody (A93786)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-CBL (phospho Tyr774) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Western Blot - Anti-CBL (phospho Tyr774) Antibody (A93786)

Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30' using Anti-CBL (phospho Tyr774) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.