This antibody recognizes CD109, an approximately 165 kDa GPI-anchored extracellular protein expressed mainly on various hematopoietic cells, activated T lymphoblasts, activated platelets, and endothelial cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
WERI-RB-1 retinoblastoma cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
W7C5
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
165 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
150 kDa TGF-beta-1-binding protein, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7, CD109 antigen, CPAMD7, p180, Platelet-specific Gov antigen, r150
Flow cytometric analysis of human PHA stimulated peripheral blood mononuclear cells stained with Anti-CD109 Antibody [W7C5] (PE) (10µl reagent per milion cells in 100µl of cell suspension).
Separation of human CD109 positive cells (red-filled) from CD109 negative cells (black-dashed) in flow cytometry analysis of human PHA stimulated peripheral blood mononuclear cells using Anti-CD109 Antibody [W7C5] (PE) (10µl reagent per milion cells in 100µl of cell suspension).
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Proteins predicted to interact with CD109
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
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