Western Blot - Anti-CD13 Antibody [ARC53706] (A306365)
Western blot analysis of extracts of various cell lines, using Anti-CD13 Antibody [ARC53706] (A306365) at 1:40,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-CD13 Antibody [ARC53706] (A306365) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-CD13 Antibody [ARC53706] (A306365) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-CD13 Antibody [ARC53706] (A306365) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD13 Antibody [ARC53706] (A306365) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow cytometry analysis of HepG2 cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-CD13 Antibody [ARC53706] (A306365), (2.5 µg/ml orange line), followed by Alexa Fluor? 647 conjugated goat anti-mouse polyclonal antibody (1:600) staining. Non-fluorescently stained HepG2 cells, used as blank control (red line).
Flow cytometry analysis of Jurkat cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-CD13 Antibody [ARC53706] (A306365), (2.5 µg/ml orange line), followed by Alexa Fluor 647 conjugated goat anti-mouse polyclonal antibody (1:600). Non-fluorescently stained Jurkat cells, used as blank control (red line).