This antibody recognises mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150 kDa subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.Rat anti Mouse CD13 antibody, clone R3-63 has been reported to block mouse APN enzyme activity.
Flow Cytometry: 1:200 - 1:1,000, Use 10µl of the suggested working dilution to label 106 cells in 100uµl
Reactivity
Mouse
Immunogen
Mouse intestinal APN.
Host
Rat
Clonality
Monoclonal
Clone ID
R3-63
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat anti Mouse CD13 antibody, clone R3-63 recognizes mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150 kDa subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.Rat anti Mouse CD13 antibody, clone R3-63 has been reported to block mouse APN enzyme activity (Hansen et al. 1993).
Synonyms
Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP-M, AP-N, APN, gp150, hAPN, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, PEPN