Anti-CD14 Antibody (A320050)

Anti-CD14 Antibody

Goat polyclonal antibody to CD14.

This antibody recognises human CD14, a 55 kDa receptor for endotoxin and other bacterial cell wall components.Goat anti Human CD14 antibody detects an epitope within the internal region of CD14. CD14 acts as a multiligand pattern recognition receptor, inducing specific coassembly of additional receptors, including MD-2 and TLR4 as part of the lipopolysaccharide (LPS) receptor.The LPS receptor is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes, and is also expressed by most tissue macrophages. Activation of the LPS receptor leads to NF-kappa-B activation, cytokine secretion and the inflammatory response. CD14 also up-regulates cell surface molecules, including adhesion molecules, and has been shown to bind apoptotic cells

WB, IHC-P, ELISA

ELISA: 1:128,000, IHC-P: 4 - 6 µg/ml, WB: 1 - 3 µg/ml

Human

A peptide sequence of C-KRVDADADPRQYAD corresponding to amino acid residues 71-84 of the human CD14 precursor conjugated to a carrier protein.

C-KRVDADADPRQYAD

Goat

Polyclonal

IgG

500 µg/ml

Approximately 55kD in human lung, lymph node and tonsil lysates.

Liquid

Supplied in TRIS Buffered Saline, 0.5% BSA and 0.02% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Goat anti Human CD14 antibody recognizes human CD14, a 55 kDa receptor for endotoxin and other bacterial cell wall components.Goat anti Human CD14 antibody detects an epitope within the internal region of CD14. CD14 acts as a multiligand pattern recognition receptor, inducing specific coassembly of additional receptors, including MD-2 and TLR4 as part of the lipopolysaccharide (LPS) receptor.The LPS receptor is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes, and is also expressed by most tissue macrophages. Activation of the LPS receptor leads to NF-kappa-B activation, cytokine secretion and the inflammatory response. CD14 also up-regulates cell surface molecules, including adhesion molecules, and has been shown to bind apoptotic cells

Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein