Anti-CD14 Antibody [B-A8] (A86083)

Anti-CD14 Antibody [B-A8]

Mouse monoclonal [B-A8] antibody to CD14.

This antibody reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages.

ELISA, IHC-P, Flow Cytometry

ELISA: This antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody MEM-18 (cat. no. 1B-212-C100), Flow Cytometry: 1 µg/ml;IHC-P: 2-10 µg/ml, Positive Control: PML brain sections; positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels. Heat retrieval of antigen is recommeded.

Human

Human monocytes.

Mouse

Monoclonal

B-A8

IgG1

Purified by differential precipitation and solid-phase chromatography.

1 mg/ml

53-55 kDa

Liquid

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein