This antibody reacts with CD14, a 53-55 kDa GPI-linked extracellular membrane glycoprotein expressed on monocytes, macrophages, and weakly on granulocytes. This antibody also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
Applications
Flow Cytometry, IP
Dilutions
IP: Excellent for immunoprecipitation of CD14, Flow Cytometry: 4 µg/ml.
Reactivity
Human, Non-Human Primates
Immunogen
A crude mixture of human urinary proteins, precipitated by ammonium sulphate, from the urine of a patient suffering from proteinuria.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-15
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
53-55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Separation of human monocytes (red-filled) from CD14 negative lymphocytes (black-dashed) in flow cytometry surface staining of peripheral whole blood, labeling CD14 with Anti-CD14 Antibody [MEM-15] (A85583), (concentration in sample 0,6 µg/ml, GAM APC).
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