Anti-CD14 Antibody [UCHM1] - BSA and Azide free (A281875)

Anti-CD14 Antibody [UCHM1] - BSA and Azide free

Mouse monoclonal [UCHM1] antibody to CD14.

This antibody recognises a cell surface antigen of ~55 kDa, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative. UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.

IHC-Fr, IF, Flow Cytometry, IP, Functional Assays

Flow Cytometry: 1:50, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Human, Cynomolgus monkey, Rhesus Monkey, Fish, Trout

Human Thymocytes followed by peripheral blood mononuclear cells.

Mouse

Monoclonal

UCHM1

IgG2a

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Human CD14 antibody, clone UCHM1 recognizes a cell surface antigen of ~55 kDa, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative (Hogg et al. 1984). UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.

Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein