This antibody recognizes an extracellular epitope of CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells, and activated keratinocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Rabbit, Canine, Mouse, Human
Cross Reactivity
This antibody does not cross react with Rat.
Immunogen
Cultured human umbilical cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
P1H12
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
118 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of cells stained with Anti-CD146 Antibody [P1H12] (PE) (A85907), (10 µl reagent per million cells in 100 µl of cell suspension, red-filled) from cells stained using Mouse IgG1 kappa Isotype Control [MOPC-21] (PE) (A86199), (concentration in sample 1,67 µg/ml, same as CD146 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of HUVEC cell suspension.
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