Mouse monoclonal [MEM-M6/1] antibody to CD147 (Alexa Fluor® 488).
Specificity
This antibody recognizes an extracellular epitope in the N terminal Ig domain of CD147, a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets, and endothelial cells. It is not expressed by resting lymphocytes. This antibody is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.
Applications
Flow Cytometry, WB
Dilutions
Flow Cytometry: This reagent is designed for analysis of human blood cells using 4µl reagent / 100µl of whole blood or 106 cells in a suspension.
Reactivity
Human
Immunogen
Purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2, and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer).
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-M6/1
Isotype
IgG1
Conjugate
Alexa Fluor® 488
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Alexa Fluor® 488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 15mM Sodium Azide.