Mouse monoclonal [OX-47] antibody to CD147 (FITC).
Specificity
This antibody recognises a membrane glycoprotein of ~42-48 kDa expressed at high levels on activated lymphocytes but lower levels on resting cells. CD147 is also present on some endothelia. The OX-47 antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue. CD147 is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7. Mouse anti Rat CD147 antibody, clone OX-47 is suitable for use in Western blotting.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Rat
Immunogen
T blasts from a mixed lymphocyte reaction.
Host
Mouse
Clonality
Monoclonal
Clone ID
OX-47
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Rat CD147 antibody, clone OX-47 recognizes a membrane glycoprotein of ~42-48 kDa expressed at high levels on activated lymphocytes but lower levels on resting cells (Paterson et al. 1987). CD147 is also present on some endothelia (Fossum et al. 1991). The OX-47 antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue (Fossum et al. 1991).CD147 is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.Mouse anti Rat CD147 antibody, clone OX-47 is suitable for use in Western blotting (Foda et al. 2001).