This antibody reacts with an epitope on CD16 antigen that is residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). This antibody reacts with CD16+ granulocytes.
Applications
Flow Cytometry, IP, WB, Functional Studies
Dilutions
FUNC: This antibody blocks binding of human IgG to Fc?RIII, Flow Cytometry: 5-10 µg/ml; Positive Control: PBL (peripheral blood lymphocytes). The antibody MEM-154 does not react with CD16a present on NK cells in many subjects, WB: Non-reducing conditions.
Reactivity
Human
Immunogen
Human granulocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-154
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, without Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CD16-II, CD16a, CD16a antigen, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FCG3, FcgammaRIIIA, FCGR3, FCGR3A, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, IgG Fc receptor III-A, Low affinity immunoglobulin gamma Fc region receptor III-A