Mouse monoclonal [SJ25C1] antibody to CD19 (PerCP).
Applications
Flow Cytometry (Cell Surface)
Positive Control
Human peripheral blood mononuclear cells (PBMCs).
Dilutions
Flow Cytometry: 5 µL/million cells in 100 µL.
Reactivity
Human
Host
Mouse
Clonality
Monoclonal
Clone ID
SJ25C1
Isotype
IgG1
Light Chains
kappa
Conjugate
PerCP
Excitation: 482nm, Emission: 675nm
Concentration
100 µg/mL
Predicted MW
95 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD19 Antibody [SJ25C1] (CF®700) (). Fluorescence was detected in the R712 (APC-700) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD19 Antibody [SJ25C1] (CF®405M) (blue). Fluorescence was detected in the V450 (Pacific Blue) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD19 Antibody [SJ25C1] (CF®488A) (green). Fluorescence was detected in the B525 (FITC) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.
Representative flow cytometry analysis of lymphocyte-gated PBMCs stained with Anti-CD19 Antibody [SJ25C1] (R-PE) ( Orange). Unstained lymphocyte-gated PBMCs (gray) served as the negative control. Fluorescence was detected in the Y585 (PE) channel.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD19 Antibody [SJ25C1] (CF®575) (orange). Fluorescence was detected in the Y585 (PE) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD19 Antibody [SJ25C1] (CF®700) (teal). Fluorescence was detected in the R712 (APC-700) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD19 Antibody [SJ25C1] (RPE-Astral™616) (red). Fluorescence was detected in the B610 (PI) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD19 Antibody [SJ25C1] (CF®647Plus) (pink). Fluorescence was detected in the R660 (APC) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD19 Antibody [SJ25C1] (APC-Astral™813) (teal). Fluorescence was detected in the R840 channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD19 Antibody [SJ25C1] (PerCP) (magenta). Fluorescence was detected in the B690 (PerCP) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.