This antibody reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
Applications
Flow Cytometry, IP
Dilutions
Flow Cytometry: 1-5 µg/ml.
Reactivity
Bovine, Human
Immunogen
Raji human Burkitt's lymphoma cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-97
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
33-37 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1
Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-CD20 rabbit polyclonal antibody. Lane 1: Original lysate of RAJI cells. Lane 2: immunoprecipitate by Mouse IgG1 Isotype Control [PPV-06] (A86755). Lane 3: Original lysate of RAJI cells. Lane 4: Immunoprecipitate by Anti-CD20 Antibody [MEM-97] (A85851).
Separation of human CD20 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CD20 with Anti-CD20 Antibody [MEM-97] (A85851), (concentration in sample 1.6 µg/ml) GAM APC.
Flow cytometry surface staining of human, canine and porcine B lymphocytes using Anti-CD79a Antibody [HM57] (A86609) and Anti-CD20 Antibody [MEM-97] - BSA and Azide free (A85848).
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