Rabbit polyclonal antibody to CD227 / MUC1 (Phospho-Tyr1229)
Specificity
The antibody detects endogenous levels of CD227 / MUC1 only when phosphorylated at tyrosine 1229.
Applications
WB, IHC, IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 1229 S-P-Y(p)-E-K) derived from Human CD227 / MUC1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
Breast carcinoma-associated antigen DF3, CA 15-3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, MUC-1, Mucin-1, PUM
Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody #11707.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody #11707 (left)or the same antibody preincubated with blocking peptide (right).
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