Mouse monoclonal [204.12] antibody to CD28 (Biotin).
Applications
Flow Cytometry, Functional Studies, IF
Positive Control
Jurkat cells or human lymph nodes and tonsil tissue.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 0.5-1 µg/mL. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human, Mouse, Monkey, Porcine, Bovine, Sheep
Immunogen
Stimulated human leukocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
204.12
Isotype
IgG2a
Light Chains
kappa
Conjugate
Biotin
Concentration
0.1 mg/mL
Predicted MW
44 kDa (monomer); 88 kDa (dimer)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.