Mouse monoclonal [C28/1636] antibody to CD28 (Biotin).
Applications
Flow Cytometry
Positive Control
Jurkat cells or human lymph nodes and tonsils.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 0.5-1 µg/mL. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Immunogen
Recombinant full-length human CD28 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
C28/1636
Isotype
IgG1
Conjugate
Biotin
Concentration
0.1 mg/mL
Predicted MW
44 kDa (monomer); 88 kDa (dimer)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.