This antibody reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
Applications
Flow Cytometry, IP
Dilutions
Flow Cytometry: 2-5 µg/ml.
Reactivity
Rat
Immunogen
Mouse A20J B lymphoma cells transfected with rat CD28.
Host
Mouse
Clonality
Monoclonal
Clone ID
JJ319
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
44 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Separation of rat CD28 positive splenocytes (red-filled) from CD28 negative splenocytes (black-dashed) in flow cytometry surface staining of rat splenocytes suspension, labeling CD28 with Anti-CD28 Antibody [JJ319] (A86347), (concentration in sample 4 µg/ml) GAM APC.
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Recombinant human monoclonal antibody to CD28 for use as a research grade Anti-Human CD28 [FR-104] b for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
Recombinant human monoclonal antibody to CD28 for use as a research grade Anti-Human CD28 [FR-104] b for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
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