Anti-CD28 (phospho Tyr218) Antibody (A94935)

Name: Anti-CD28 (phospho Tyr218) Antibody
Brand: Antibodies.com
SKU: A94935
Price: 115.0 GBP
Availability: InStock

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This product is discontinued

Anti-CD28 (phospho Tyr218) Antibody (A94935) has been discontinued and is no longer available.

View all CD28 Antibodies.

Specifications

Name

Anti-CD28 (phospho Tyr218) Antibody

Description

Rabbit polyclonal antibody to CD28 (phospho Tyr218).

Specificity

This antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.

Applications

WB, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:10000

Reactivity

Human, Mouse

Immunogen

Synthetic phospho-peptide derived from human CD28 around the phosphorylation site of Tyr218.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

25kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

T-cell-specific surface glycoprotein CD28, TP44

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Anti-CD28 (phospho Tyr218) Antibody (A94935)

ELISA for immunogen phosphopeptide (left) and non-phosphopeptide (right) using Anti-CD28 (phospho Tyr218) Antibody.

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Western Blot - Anti-CD28 (phospho Tyr218) Antibody (A94935)

Western blot analysis of lysates from HepG2 cells treated with nocodazole 1µg/ml 16h using Anti-CD28 (phospho Tyr218) Antibody.

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