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Anti-CD28 (phospho Tyr218) Antibody (A94935)

ELISA - Anti-CD28 (phospho Tyr218) Antibody (P12-1088) - Antibodies.com Western Blot - Anti-CD28 (phospho Tyr218) Antibody (P12-1088) - Antibodies.com
This product is discontinued

Anti-CD28 (phospho Tyr218) Antibody (A94935) has been discontinued and is no longer available.

View all CD28 Antibodies.

  • Specifications

Name
Anti-CD28 (phospho Tyr218) Antibody
Description
Rabbit polyclonal antibody to CD28 (phospho Tyr218).
Specificity
This antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.
Applications
WB, ELISA
Dilutions
WB: 1:500-1:1000, ELISA: 1:10000
Reactivity
Human, Mouse
Immunogen
Synthetic phospho-peptide derived from human CD28 around the phosphorylation site of Tyr218.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
25kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
T-cell-specific surface glycoprotein CD28, TP44
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

ELISA - Anti-CD28 (phospho Tyr218) Antibody (P12-1088) - Antibodies.comEnlarge Image

Anti-CD28 (phospho Tyr218) Antibody (A94935)

ELISA for immunogen phosphopeptide (left) and non-phosphopeptide (right) using Anti-CD28 (phospho Tyr218) Antibody.
Western Blot - Anti-CD28 (phospho Tyr218) Antibody (P12-1088) - Antibodies.comEnlarge Image

Western Blot - Anti-CD28 (phospho Tyr218) Antibody (A94935)

Western blot analysis of lysates from HepG2 cells treated with nocodazole 1µg/ml 16h using Anti-CD28 (phospho Tyr218) Antibody.

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Proteins predicted to interact with CD28

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score
99.8% Relevancy Score
99.8% Relevancy Score
99.7% Relevancy Score
99.7% Relevancy Score
99.7% Relevancy Score