This antibody recognises bovine CD3. CD3 plays an important role in T cell activation and in expression of intact TCR molecules. Clone MM1A detects bovine CD3 and reacts with bovine alpha beta and gamma delta TCR+ T cells and recognises an epitope located on the epsilon chain of CD3. Mouse anti Bovine CD3, clone MM1A also reacts with Zebu.
Applications
Flow Cytometry, ICC
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Bovine, Bison, Water Buffalo
Immunogen
Monocyte enriched bovine mononuclear cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
MM1A
Isotype
IgG1
Conjugate
Unconjugated
Purification
0.2µM sterile filtered.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in serum free tissue culture medium containing proprietary protein free supplement and 0.09% Sodium Azide.
Storage
Store at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Bovine CD3, clone MM1A, recognizes bovine CD3. CD3 plays an important role in T cell activation and in expression of intact TCR molecules. Clone MM1A detects bovine CD3 and reacts with bovine alpha beta and gamma delta TCR+ T cells and recognizes an epitope located on the epsilon chain of CD3 (MacHugh et al. 1998). Mouse anti Bovine CD3, clone MM1A also reacts with Zebu (Conrad et al. 2007).
Flow cytometric analysis of bovine peripheral blood lymphocytes. A. Mouse IgG1 - Isotype Control (A284021) followed by Goat Anti-Mouse IgG1 Antibody (PE) (A282649). B. Anti-CD3 epsilon Antibody [MM1A] (A281228) followed by Goat Anti-Mouse IgG1 Antibody (PE) (A282649).Both the Mouse IgG1 negative control and Mouse anti Bovine CD3 are plotted against bovine CD4 as recognized by Anti-CD4 Antibody [CC8] (FITC) (A280063).
Publishing research using Anti-CD3 epsilon Antibody [MM1A] (A281228)? Please let us know so that we can list the citation on this page.
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